Coding

Part:BBa_K364202:Design

Designed by: Endre Károly Kristóf   Group: iGEM10_Debrecen-Hungary   (2010-08-10)

hBAX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compatible with RFC-10 and RFC-25.


Source

Human BAX (of the Bcl-2 gene family). Arteficially synthetized and then placed into pSB1C3 carrier vector.

References

Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 1993 Aug 27;74(4):609-19.

Vieira HL, Haouzi D, El Hamel C, Jacotot E, Belzacq AS, Brenner C, Kroemer G. Permeabilization of the mitochondrial inner membrane during apoptosis: impact of the adenine nucleotide translocator. Cell Death Differ. 2000 Dec;7(12):1146-54.

Wolter, K. G.; Hsu, Y., Smith, C. L., Mechushtan, A., Xi, X., and Youle, R. J. (December 1997). "Movement of BAX from Cytosol to Mitochondria during Apoptosis". Journal of Cell Biology 139 (5): 1281–1292. doi:10.1083/jcb.139.5.1281. PMID 9382873. PMC 2140220. http://www.jcb.org/cgi/content/full/139/5/1281.